This study explores determining the sex of humans from blood stains taken from different surfaces and compares the time course\nof detection with the conventional PCR, Conventional LoopMediated Isothermal Amplification (LAMP), and LAMP-Lateral Flow\nDipstick (LFD). For the DNA templates, 7 male and 7 female blood stained samples were extracted and added to LAMP and PCR\nreaction solution to amplify the SRY gene. The DNA samples were extracted from the following blood stained materials: cloth,\nwood, clay, and tile. Then, the samples were stored at roomtemperature for 1, 7, 30, and 60 day(s). After the DNA amplification, the\ngel electrophoresis process was applied to detect LAMP product.The LFD was combined with the LAMP to detect LAMP product\non the male cloth samples. For the male samples, the time course of detection on the first and seventh days indicated positive for\nboth LAMP and PCR products on all the surfaces while no DNA amplification was found on any of the female samples. On day 30,\npositive LAMP product was still found on all the male samples. However, it had faded on the tiles.Moreover, all the male samples,\nwhich had tested positive for PCR product, were blurred and unclear. On day 60, LAMP product was still found on all the male\nsamples. Conversely, the PCR method resulted in no bands showing for any of themale samples. However, the LAMP-LFD method\ndetected product on all the male samples of cloth.The results show that the LAMP is an effective, practical, and reliable molecularbiological\nmethod. Moreover, the LFD can increase the efficiency and sensitivity of the LAMP, making it more suitable for field\nstudies because gel electrophoresis apparatus is not required.
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